Quantification and Isolation of Spontaneous Colony Growth Variants

Methods Mol Biol. 2022:2391:55-62. doi: 10.1007/978-1-0716-1795-3_5.

Abstract

The appearance of colony growth sectors on solid medium plates has been described in many fungi. Although the molecular bases of this phenomenon remain largely unknown, possible relationships with genetic or epigenetic changes have been reported. Here we present a method to quantify the frequency of colony growth sectors in Fusarium oxysporum, which can be used to compare different fungal strains and to infer their genetic instability.

Keywords: Colony sectorization; Growth variant; Phenotypic plasticity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Culture Media
  • Fusarium / genetics*

Substances

  • Culture Media

Supplementary concepts

  • Fusarium oxysporum